pyogenes on toothbrushes.Ī placa bacteriana que se adere aos dentes e a saburra lingual servem como reservatório de patógenos que podem desenvolver infecções na cavidade bucal e de modo sistêmico. The vinegar solution reduced the presence of S. Solutions based on triclosan-containing dentifrice may be considered effective, nontoxic, cost-effective, and an easily applicable alternative for the disinfection of toothbrushes. The sodium perborate solution was the less effective against the tested microorganisms. As to the disinfection with 50% vinegar, a significant reduction was observed for all the microorganisms, except for C. The triclosan-based dentifrice solution promoted a significant reduction of all microorganisms' counts in relation to the control group. The chlorhexidine digluconate solution was the most effective disinfectant. The values of cfu/toothbrush of each group of microorganism after disinfection were compared by Kruskal-Wallis ANOVA and Dunn's test for multiple comparisons (5%). After the disinfection procedure, the number of remaining microbial cells was evaluated. The disinfection method was immersion in the disinfectant for 10 min. The following disinfectants were tested: 0.12% chlorhexidine digluconate, 50% white vinegar, a triclosan-containing dentifrice solution, and a perborate-based tablet solution. The toothbrushes were contaminated in vitro by standardized suspensions of Streptococcus mutans, Streptococcus pyogenes, Staphylococcus aureus or Candida albicans. The toothbrushes were divided into 20 experimental groups (n = 10), according to microorganism considered and chemical agent used. Two-hundred toothbrushes with standardized dimensions and bristles were included in the study. The aim of this study was to evaluate alternative methods for the disinfection of toothbrushes considering that most of the previously proposed methods are expensive and cannot be easily implemented.
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